Human recombinant cIAP2-BIR3.
ACCESSION #: NM_001165.4
Uniprot Link
INCLUDES AMINO ACIDS: 244-349
TAG(S): N-terminal GST tag C-terminal StrepII
MW: 40.2 kDa
EXPRESSION SYSTEM: E.coli
PURITY: >90% by SDS-PAGE
ASSAY CONDITIONS: RBC’s cIAP2-BIR3 domain displays binding affinity for Smac/DIABLO peptide. Reactions were performed with 20nM FAM-labelled peptide at room temperature in 50mM HEPES, pH 7.5, 200mM NaCl, 0.01% Triton X100. The resulting polarization values (Ex. 480 nm/Em. 535 nm) was read following a 30 minute incubation.(See figure, below.)
SUPPLIED AS: _ µg/µL in 50 mM Tris HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted enzyme is not recommended.
AMOUNT: 100ug
