Human recombinant cIAP-BIR3.
ACCESSION #: NM_001166.4
Uniprot Link
INCLUDES AMINO ACIDS: 258-363
TAG(S): N-terminal His tag and C-terminal StrepII tag
MW: 16.0 kDa
EXPRESSION SYSTEM: E.coli
PURITY: >85% by SDS-PAGE
ASSAY CONDITIONS: RBC’s cIAP1-BIR3 domain displays binding affinity for Smac/DIABLO peptide. Reactions were performed with 20nM FAM-labelled peptide at room temperature in 50mM HEPES, pH 7.5, 200mM NaCl, 0.01% Triton X100. The resulting polarization values (Ex. 480 nm/Em. 535 nm) was read following a 30 minute incubation. (See figure, below.)
SUPPLIED AS: _ µg/µL in 50 mM Tris HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted enzyme is not recommended.
AMOUNT: 100ug
