Mutant human recombinant NRAS (Q61H) with histidine (H) substituted for glutamine-61 (Q61)
ACCESSION #: NP_002515.1
INCLUDES AMINO ACIDS: residues 2-169
TAG(S): N-terminal GST-fusion protein
MW: 45.9 kDa
EXPRESSION SYSTEM: E. coli
PURITY: >95% by SDS-PAGE
SUPPLIED AS: Solution of purified recombinant protein in 25 mM Tris, pH 8.0, 500 mM NaCl, 5% glycerol, 5mM MgCl2,1 mM TCEP.
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry ice/ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted enzyme is not recommended.
