Human recombinant JARID1B. Substrate peptide: B-H3K4Me3 quantified product: B-H3K4Me2 Detection method: HTRF (Cisbio)
ACCESSION #: NM_006618.3
INCLUDES AMINO ACIDS: 2-752
TAG(S): N-terminal FLAG tag
MW: 90.7 kDa
EXPRESSION SYSTEM: Sf21 insect cells
SUPPLIED AS: µg/µL in 50 mM Tris HCl, pH 7.5, 150 mM NaCl, 10% glycerol, 1 mM TCEP
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.