Full-length, untagged human recombinant histone H3.3 and full-length, untagged human recombinant histone H4.
ACCESSION #: NM_002107 (H3.3), NM_003538 (H4)
Uniprot Link 1
Uniprot Link 2
INCLUDES AMINO ACIDS: 2-136 (H3.3), 2-103 (H4)
TAG(S): None
MW: 15.3 kDa (H3.3), 11.1 kDa (H4)
EXPRESSION SYSTEM: E.coli
SUPPLIED AS: Solution of purified recombinant protein in 10 mM Tris HCl, pH 7.5, 500 mM NaCl, 5% glycerol and 5mM 2-mercaptoethanol
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.