Human recombinant EZH2 in complex with human recombinants EED. Total complex MW is 228.7 kDa. All proteins are full-length (residue 2 through C-terminus) and co-expressed in an insect cell/baculovirus expression system. The EED subunit incorporates an N-terminal Strep and Flag-tag and all others include an N-terminal His-tag. Catalyzes the transfer of methyl groups from S-adenosyl-L-methionine (SAM) to the e-amino function of protein L-lysine residues, specifically lysine-27 of histone H3 (H3K27). During development, Polycomb Repressive Complex 2 (PRC2) is the principal methyltransferase responsible for generating trimethylated histone H3 lysine-27 (H3K27me3), an epigenetic mark essential for programmed repression of gene expression1-5. EZH2, which includes a SET methyltransferase domain, is the catalytic subunit of PRC21,6. The core of the catalytic complex includes EZH2, EED, SUZ12 and RbAp48, while addition of AEBP2 significantly enhances the methyltransferase activity of the complex (>3x)6. EZH2 is overexpressed in a wide range of human cancers and its overexpression can correlate with tumor progression, increased metastasis and poor prognosis (see review7). Depletion of EZH2 and/or other PRC2 components can inhibit growth or induce apoptosis in cancer cells6, 8-10. Consequently, EZH2 is considered a promising target for the development of anti-cancer therapies11

ACCESSION #: EZH2 NM_001203247, EED NM_003797, SUZ12 NM_015355

INCLUDES AMINO ACIDS: EZH2 2-746, EED 2-441, SUZ12 2-739

TAG(S): EED has an N-terminal strep and Flag-tag, all others N-terminal His-tag

MW: 228.7 kDa

EXPRESSION SYSTEM: Insect cell/Baculovirus

SUPPLIED AS: Solution of purified recombinant protein in 20 mM Tris-HCl, pH 8, 150 mM NaCl, 2 mM MgCl₂, 2 mM DTT, 20% glycerol (w/v), 0.01% NP-40 as determined by OD₂₈₀.

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.