Human recombinant EZH1 (residues 2-747; Genbank Accession # NM_001991; MW = 88 kDa) in complex with human recombinants AEBP2 (2-517; NM_001114176; 57 kDa), EED (2-441; NM_003797; 51 kDa), RbAp48 (2-425; NM_005610; 51 kDa) and SUZ12 (2-739; NM_015355; 86 kDa). Total complex MW is 333.4 kDa. All proteins are full-length (residue 2 through C-terminus) and co-expressed in an insect cell/baculovirus expression system. The EED subunit incorporates an N-terminal Flag-tag and all others include an N-terminal His-tag. Catalyzes the transfer of methyl groups from S-adenosyl-L-methionine (SAM) to the e-amino function of protein L-lysine residues, specifically lysine-27 of histone H3 (H3K27). During development, Polycomb Repressive Complex 2 (PRC2) is the principal methyltransferase responsible for generating trimethylated histone H3 lysine-27 (H3K27me3), an epigenetic mark essential for programmed repression of gene expression1-5. EZH2, which includes a SET methyltransferase domain, functions as the catalytic subunit of PRC21,6, but can be replaced by the homologous EZH17 , which is less catalytically active8,9, although PRC2-Ezh1 has strong repressive activity on transcription independent of its methyltransferase activity9. EZH1 acts to preserve the repressive H3K27me3 mark in embryonic stem cells, complementing the role of EZH28. Multiple recent results suggest important roles for EZH1 in developmental and homeostatic processes, including maintenance of the left-right axis10, hair follicle homeostasis and wound repair11, skeletal muscle differentiation12 and adult hematopoiesis13.

ACCESSION #: NM_001991, NM_001114176, NM_003797, NM_005610, NM_015355

SUPPLIED AS: __ µM EZH1 Complex, as defined above, (___ µg/µl total protein) in 20 mM Tris-HCl, pH 7.9, 150 mM NaCl, 2 mM MgCl2, 2 mM DTT, 20% glycerol (w/v), 0.01% NP-40.

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted enzyme should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted enzyme is not recommended.