Human recombinant EED expressed in an insect cell/baculovirus expression system with an N-terminal Strep-Flag tag. EED functions as H3K27me3 reader within the PRC2/EED-EZH2 complex and mediates protein-protein interactions. During development, Polycomb Repressive Complex 2 (PRC2) is the principal methyltransferase responsible for generating trimethylated histone H3 lysine-27 (H3K27me3), an epigenetic mark essential for programmed repression of gene expression1-5.

ACCESSION #: NM_003797

INCLUDES AMINO ACIDS: 2-441

TAG(S): N-terminal Strep-Flag tag

MW: 53.9 kDa

EXPRESSION SYSTEM: Insect Cell/Baculovirus

SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris-HCl, pH 8, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v).

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.