Mutant apoptosis regulator Bcl-2 (F104L).

ACCESSION #: NM_000633.3

INCLUDES AMINO ACIDS: wildtype residues 1-207; leucine (L) substituted for phenylalanine-104 (F)

TAG(S): C-terminal GST fusion protein

MW: 49.2 kDa

EXPRESSION SYSTEM: E. coli

PURITY: > 95% by SDS-PAGE

SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris, pH 7.5, 500 mM NaCl, 10% glycerol, 0.25 mM TCEP.

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.