BIX-01294 is a selective inhibitor of G9a histone methyltransferase (G9aHMTase; IC₅₀ = 1.7 µM) as well as GLP HMTase (IC₅₀ = 38 µM) leading to a decrease in H3K9Me2 (histone H3 lysine 9 methylation) in vitro. It facilitates the reactivation of pluripotency genes and induces passive demethylation, thus promoting reprogramming.BIX-01294, in combination with BAY K8644 (a calcium channel agonist), was found to improve reprogramming efficiencies of Oct4-Klf4-(OK)-infected neural progenitor cells. It induced apoptosis and decreased proliferation, mobility and invasion in human neuroblastoma cells.

Physical Properties: Off-white crystalline powder

MW=600.0 C₂₈H₃₈N₆O₂ · 3HCl

Analysis: >98% (HPLC); NMR (Conforms)

Solubilization: May be dissolved in water (50 mg/ml); or DMSO (50 mg/ml)

Storage: Store desiccated as supplied at ambient for up to 2 years. Store solutions at-20°C for up to 3 months.

Amount: 5mg