Human recombinant cIAP-BIR3.
ACCESSION #: NM_001165.4
INCLUDES AMINO ACIDS: 244-349
TAG(S): N-terminal His tag and C-terminal StrepII
EXPRESSION SYSTEM: E.coli
PURITY: >80% by SDS-PAGE
RBC’s cIAP2-BIR3 domain displays binding
affinity for Smac/DIABLO peptide. Reactions were performed with 20nM
FAM-labelled peptide at room temperature in 50mM HEPES, pH 7.5, 200mM NaCl,
0.01% Triton X100. The resulting polarization values (Ex. 480 nm/Em. 535 nm)
was read following a 30 minute incubation.
(See figure, below.)
SUPPLIED AS: _ µg/µL in 50 mM Tris HCl, pH 7.5,
500 mM NaCl, 1 mM TCEP, 10% glycerolSTORAGE: -70°C. Thaw quickly and store on ice before use.
The remaining, unused, undiluted protein should be snap frozen,
for example in a dry ice ethanol bath or liquid nitrogen.Minimize freeze/thaws if possible, but very
low volume aliquots (<5 µl) or storage of diluted enzyme is not recommended.
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Substantial Discounts Available on Bulk Quantities