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Your Price:$303.00
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Part Number:APT-11-373

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Human recombinant cIAP2-BIR3.

ACCESSION #: NM_001165.4

Uniprot Link


TAG(S): N-terminal GST tag C-terminal StrepII

MW: 40.2 kDa



ASSAY CONDITIONS: RBC’s cIAP2-BIR3 domain displays binding affinity for Smac/DIABLO peptide. Reactions were performed with 20nM FAM-labelled peptide at room temperature in 50mM HEPES, pH 7.5, 200mM NaCl, 0.01% Triton X100. The resulting polarization values (Ex. 480 nm/Em. 535 nm) was read following a 30 minute incubation.  (See figure, below.)

SUPPLIED AS:  _ µg/µL in 50 mM Tris HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry ice ethanol bath or liquid nitrogen.  Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted enzyme is not recommended.

AMOUNT: 100ug

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With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

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