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XIAP-BIR3 (His)

Part Number:APT-11-374
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Human recombinant XIAP-BIR3.

ACCESSION #: NM_001167.3

Uniprot Link

INCLUDES AMINO ACIDS: 255-356

TAG(S): N-terminal His tag C-terminal StrepII tag

MW: 15.8 kDa

EXPRESSION SYSTEM: E.coli

PURITY: >95% by SDS-PAGE

ASSAY CONDITIONS: RBC’s XIAP-BIR3 domain displays binding affinity for Smac/DIABLO peptide. Reactions were performed with 20nM FAM-labelled peptide at room temperature in 50mM HEPES, pH 7.5, 200mM NaCl, 0.01% Triton X100. The resulting polarization values (Ex. 480 nm/Em. 535 nm) was read following a 30 minute incubation.(See figure, below.)


SUPPLIED AS: _ µg/µL in 50 mM Tris HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted enzyme is not recommended.

AMOUNT: 100ug

 
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