The DNA damage response protein 53BP1 utilizes its tandem tudor domain to recognize dimethylated lysine 20 on histone (H4K20me2), a modification associated with double-strand DNA breaks. UNC-2170, identified by screening, was found to be a uM ligand for 53BP1 which also demonstrated at least 17-fold selectivity for 53BP1 over other methyllysine binding proteins. The compound functions as a 53BP1 antagonist in cell lysates and suppresses class switch recombination in whole cells, a process requiring a functioning 53BP1 tudor domain.
Physical Properties: White powder
MW=349.7 C14H21BrN2O• HCl
Analysis: >98% (TLC); NMR (Conforms)
Solubilization: May be dissolved in DMSO (40 mg/ml); or water (50 mg/ml)
Storage: Store desiccated as supplied at -20°C for up to 2 years. Store solutions at -20°C for up to 3 months.
Amount: 5 mg