Splitomicin was initially shown to inhibit Sir2p
(the yeast Sirt1 homolog) and create a conditional phenocopy of a sir2 deletion
mutant in S. cerevisiae.
Cells grown in the presence of the drug have silencing defects at telomeres,
silent mating-type loci and the ribosomal DNA. Inhibits all class
III HDACs (sirtuins) and alleviates gene silencing in Fragile X mental
retardation syndrome. Reverses ischemic preconditioning-induced
cardioprotection in a mouse model.
Physical Properties: White powder
Analysis: >98% (TLC); NMR
Solubilization: May be dissolved in DMSO (>25 mg/ml) or ethanol (>25
Storage: Store desiccated as
supplied at ambient temperature for up to 2 years. Store solutions at -20°C for
up to 1 month.