Human recombinant SMYD3 expressed with an C-terminal His-tag in Sf9 insect cells. Catalyzes the transfer of methyl groups from S-adenosyl-L-methionine (SAM) to the e-amino function of protein L-lysine residues, particularly in histones H4 and H3, although other activities have been reported. Lysine methyltransferases of the SMYD family are unusual in that their SET domains are split in two by insertion of a myeloid-Nervy-DEAF-1 (MYND) domain in the primary amino acid sequence^1-3. SMYD3 (SET and MYND domain containing protein 3) has been described as a histone H3 lysine-4 (H3K4) and histone H4 lysine-20 (H4K20) methyltransferase (HMT), although more recent evidence suggests that it may be a histone H4 lysine-5 (H4K5) HMT. Smyd3 has also been shown to methylate K260 of MAP3K2^4,5. SMYD3 is overexpressed in a number of cancers, including breast and liver.

ACCESSION #: NM_001167740

INCLUDES AMINO ACIDS: 2-428 (C-terminus)

TAG(S): C-terminal HIS-tag

MW: 50.1 kDa

EXPRESSION SYSTEM: Sf9 insect cells

SUPPLIED AS: Solution of purified recombinant protein in 20 mM Tris HCl, pH 8.0, 250 mM NaCl, 1 mM TCEP, 10% glycerol as determined by OD₂₈₀

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. Freezing and storage of diluted enzyme is not recommended.