customer service




Smyd3 (Baculo)

Your Price:$358.00
In Stock.
Part Number:HMT-21-478

Choose Options

Amount

**Please note that shipping and dry ice charges will be included in your final invoice.**

DESCRIPTION: 

Human recombinant SMYD3 expressed with an C-terminal His-tag in Sf9 insect cells. Catalyzes the transfer of methyl groups from S-adenosyl-L-methionine (SAM) to the e-amino function of protein L-lysine residues, particularly in histones H4 and H3, although other activities have been reported. Lysine methyltransferases of the SMYD family are unusual in that their SET domains are split in two by insertion of a myeloid-Nervy-DEAF-1 (MYND) domain in the primary amino acid sequence1-3. SMYD3 (SET and MYND domain containing protein 3) has been described as a histone H3 lysine-4 (H3K4) and histone H4 lysine-20 (H4K20) methyltransferase (HMT), although more recent evidence suggests that it may be a histone H4 lysine-5 (H4K5) HMT. Smyd3 has also been shown to methylate K260 of MAP3K24,5. SMYD3 is overexpressed in a number of cancers, including breast and liver.

ACCESSION #: NM_001167740

INCLUDES AMINO ACIDS: 2-428 (C-terminus)

TAG(S): C-terminal HIS-tag

MW: 50.1 kDa

EXPRESSION SYSTEM: Sf9 insect cells

SUPPLIED AS: Solution of purified recombinant protein in 20 mM Tris HCl, pH 8.0, 250 mM NaCl, 1 mM TCEP, 10% glycerol as determined by OD280

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. Freezing and storage of diluted enzyme is not recommended.

View Product Data Sheet

 

Substantial Discounts Available on Bulk Quantities











With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

Reaction Biology Corp. 1 Great Valley Parkway, Suite 2 Malvern, PA 19355 1-877-347-2368 or 610-722-0247
Sales@reactionbiology.com
Reaction Biology Corp. © 2013 all rights reserved.