Human recombinant SMYD3 expressed
with an C-terminal His-tag in Sf9 insect cells. Catalyzes the transfer of
methyl groups from S-adenosyl-L-methionine (SAM) to the e-amino function of
protein L-lysine residues, particularly in histones H4 and H3, although other
activities have been reported. Lysine methyltransferases of the SMYD family are
unusual in that their SET domains are split in two by insertion of a
myeloid-Nervy-DEAF-1 (MYND) domain in the primary amino acid sequence1-3.
SMYD3 (SET and MYND domain containing protein 3) has been described as a
histone H3 lysine-4 (H3K4) and histone H4 lysine-20 (H4K20) methyltransferase
(HMT), although more recent evidence suggests that it may be a histone H4
lysine-5 (H4K5) HMT. Smyd3 has also been shown to methylate K260 of MAP3K24,5.
SMYD3 is overexpressed in a number of cancers, including breast and liver.
ACCESSION #: NM_001167740
INCLUDES AMINO ACIDS: 2-428 (C-terminus)
TAG(S): C-terminal HIS-tag
EXPRESSION SYSTEM: Sf9 insect cells
SUPPLIED AS: Solution of purified recombinant protein in 20 mM Tris HCl,
pH 8.0, 250 mM NaCl, 1 mM TCEP, 10% glycerol as determined by OD280
STORAGE: -70°C. Thaw quickly and store on ice before use. The
remaining, unused, undiluted enzyme should be refrozen quickly by, for example,
snap freezing in a dry/ice ethanol bath or liquid nitrogen. Freezing and
storage of diluted enzyme is not recommended.
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