Human recombinant SET8 expressed in E. coli with an N-terminal GST fusion-tag. Catalyzes the transfer of methyl groups from S-adenosyl-L-methionine (SAM) to the e-amino function of protein L-lysine residues, specifically the monomethylation of lysine-20 of histone H4 (H4K20me1) and of lysine-382 of p53 (p53K382me1). SET8 and H4K20me1 are essential to chromosome condensation, entry into mitosis and maintenance of genomic stability and are implicated in the licensing of replication origins. Monomethylation of H4K20 and cellular SET8 levels oscillate with the cell cycle, peaking at the G2/M transition, declining late in mitosis, with SET8 protein becoming nearly undetectable in S phase. Apart from its role in cell cycle progression, there is evidence SET8 can regulate transcription of specific genes by H4K20 monomethylation in promoter elements or gene bodies and by methylation of a transcription factor, p53. Multiple lines of evidence suggest that SET8 may be a promising target for anti-cancer therapy. These include the requirement for SET8 in cell cycle progression, SET8’s activation of Wnt target genes, its negative regulation of the p53 tumor suppressor, the association of increased SET8 levels with breast cancer metastasis and the association of decreased levels with longer survival in hepatocellular carcinoma patients.

ACCESSION #: NM_020382

INCLUDES AMINO ACIDS: 190-352

TAG(S): N-terminal GST fusion-tag

MW: 45.4 kDa

EXPRESSION SYSTEM: E. coli

SUPPLIED AS: Solution of purified recombinant protein in 20 mM Tris HCl, pH 7.5, 300 mM NaCl, 10% glycerol (v/v), 1 mM TCEP as determined by OD_280.

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted enzyme should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted enzyme is not recommended