SET1B Complex is a complex catalyzing the transcription-activating methylation of histone H3 lysine-4 and comprising a catalytic, SET domain-containing subunit, SET1B (SETD1B), plus the subunits of an activity-enhancing sub-complex—WDR5, RbBP5, Ash2L and DPY-30 (2 copies). In contrast to the H3K4-methylating activities of other MLL/SET1 family members that are more narrowly focused (e.g. MLL1 and MLL2 and the HOX genes), human SET1-containing complexes (SET1A and SET1B) appear to be responsible for widespread H3K4 trimethylation. However, the subnuclear localizations of SET1A and SET1B appear to overlap very little, suggesting that they may regulate different sets of genes.
ACCESSION #: NM_015048, NM_017588, NM_005057, NM_001105214, NM_0325742
INCLUDES AMINO ACIDS: SET1B 1629-1923 (C-term.), WDR5 22-334 (C-term.), RbBP5 1-538 (C-term.), Ash2L 2-534 (C-term.), DPY-30 1-99 (C-term.)
TAG(S): N-terminal His-tag on all subunits, but SET1B, which is GST-tagged
MW: 249.0 kDa
EXPRESSION SYSTEM: E. coli
SUPPLIED AS: Solution of purified recombinant proteins in 20 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM TCEP (tris(2-carboxyethyl)phosphine HCl), 10% (w/v) glycerol, 1 µM ZnCl2.
STORAGE: -80°C, aliquot and snap-freeze after first use.
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