Mononucleosomes assembled from full-length human histones. Histone octamers are assembled into mononucleosomes by standard methods with a 211 bp DNA comprising a strong nucleosome positioning sequence. Each mononucleosome unit will thus contain 2 copies each of the four histones, plus the 211 bp DNA, for a total MW of 256 kDa.
NOTE: Each vial contains 50 µg nucleosomal DNA, determined by A260nm. Given the assembly ratio of 211 bp/nucleosome, the total weight, DNA + protein, is 89.7 µg.
A 50 ug vial of RBC Recombinant Nucleosomes contains over 75% more product than those from vendors that sell by total weight!
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted portion should be snap frozen, for
example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of
diluted solutions are not recommended.
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Substantial Discounts Available on Bulk Quantities