Human recombinant PRMT8. PRMT8, a type I arginine methyltransferase, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (SAM) to an w-nitrogen of the guanidino function of protein L-arginine residues (w-monomethylation) and the transfer of a second methyl group to the same nitrogen, yielding asymmetric dimethylarginine (aDMA). Although highly homologous (>80% identity) to the major arginine methyltransferase PRMT1, distinctive characteristics of PRMT8 include its unique, regulatory 76 residue N-terminal domain and its brain-specific expression, especially enhanced in the cortex. Although transfected GFP-PRMT8 can be myristoylated and localized to the plasma membrane1, other evidence suggests that the endogenous, central nervous system PRMT8 is a nuclear protein. Removal of the N-terminal 60 amino acids of PRMT8 has been shown to dramatically enhance PRMT8 catalytic activity. RBC’s PRMT8 is deleted for residues 1-60 and is highly active with the substrate GST-GAR.

ACCESSION #: NM_019854

Uniprot Link

INCLUDES AMINO ACIDS: 61-394 (C-terminus)

TAG(S): N-terminal GST fusion protein and C-terminal Strep-tag

MW: 67.09 kDa

EXPRESSION SYSTEM: Sf9 insect cells

SUPPLIED AS: 2.13 µg/µl in 50mM Tris-HCl pH 8, 500mM NaCl, 10% glycerol, 0.25mM TCEP, 2 mM DTT

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted enzyme should be refrozen quickly by, for example, snap freezing in a dry ice-ethanol bath or liquid nitrogen. Freezing and storage of diluted enzyme is not recommended.