customer service




PRMT5(C449S)/MEP50

Your Price:$879.00
In Stock.
Part Number:HMT-22-434

Choose Options

Amount

**Please note that shipping and dry ice charges will be included in your final invoice.**

DESCRIPTION:
Mutant Human recombinant PRMT5 in complex with human recombinant MEP50, produced by co-expression in an insect cell/baculovirus expression system. PRMT5, a type II arginine methyltransferase, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (SAM) to an w-nitrogen, of the guanidino function of protein L-arginine residues (w-monomethylation) and the transfer of a second methyl group to the other w-nitrogen, yielding symmetric dimethylarginine (sDMA). Structural studies show the PRMT5/MEP50 complex to consist of four heterodimers. PRMT5 catalytic activity is weak when not complexed with MEP50, which may be due to MEP50’s role in substrate binding. A component of multiple macromolecular complexes (e.g. 20S Methylosome, Swi/Snf), PRMT5/MEP50 is located in both the nucleus and cytoplasm, modifies a variety of substrates and plays roles in chromatin remodeling, RNA processing, and regulation of gene expression, cell growth and differentiation. Its pro-proliferative effects and their association with multiple cancers (lung, breast, ovarian, lymphoid) has led to increasing interest in PRMT5 as a target for anti-cancer therapy.

ACCESSION #: NM_006109, NM_024102

Uniprot Link

Uniprot Link2

INCLUDES AMINO ACIDS: PRMT5 2-637(C-term.) with cysteine substitute for serine-499, MEP50 2-342 (C-term.)

TAG(S): N-terminal Strep and Flag-tags (PRMT5), N-terminal His (MEP50)

MW: 73.7/39.9 kDa

EXPRESSION SYSTEM: Insect cell/Baculovirus

SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris/HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM TCEP, 20% (v/v) glycerol.

STORAGE: -70°C, aliquot and snap-freeze after first use.

View Product Data Sheet

http://www.reactionbiology.com/webapps/site/Images/acrobat.gif

Substantial Discounts Available on Bulk Quantities













With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

Reaction Biology Corp. 1 Great Valley Parkway, Suite 2 Malvern, PA 19355 1-877-347-2368 or 610-722-0247
[email protected]
Reaction Biology Corp. © 2013 all rights reserved.