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PRDM9 Methyltransferase

Your Price:$495.00
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Part Number:HMT-21-152

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Human recombinant PRDM9 expressed in Sf9 insect cells with an N-terminal GST-tag. Catalyzes the transfer of methyl groups from S-adenosyl-L-methionine (SAM) to the epsilon-amino function of protein L-lysine residues, specifically histone H3 lysine-4 (H3K4). Expressed only in meiotic male and female germ cells, PRDM9 is required for fully-functional pairing of homologous chromosomes, double strand break repair, meiotic progression and fertility. PRDM9 binds, via its C-terminal array of zinc fingers (ZnFs), to sequence motifs associated with “hotspots” for homologous recombination (crossovers). Such hotspots are enriched for trimethylated histone H3 lysine-4 (H3K4me3), presumably the result of PRDM9’s H3K4 methyltransferase activity, and part of PRDM9’s role may be to direct meiotic recombination events away from non-PRDM9 H3K4me3 marks, such as those in promoters. PRDM9’s histone methyltransferase activity has received relatively little study, although results with the E. coli-expressed mouse enzyme imply that it is an H3K4 trimethylase that only catalyzes the H3K4me2 to H3K4me3 transition. However, since RBC’s insect cell-expressed human enzyme is most active with, among the substrates tested, the presumably unmethylated E. coli-expressed histone H3.3 (RBC Cat. # HMT-11-134), this would suggest that it is capable of other methylations as well. 

ACCESSION #: NM_020227

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TAG(S): N-terminal GST-tag

MW: 76.3 kDa

EXPRESSION SYSTEM: Insect cell/Baculovirus

SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris/HCl pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v).

STORAGE: -80°C, aliquot and snap-freeze after first use.

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We also offer assay services for PRDM9.

With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

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