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Nucleosomes (HeLa Mono/Di)

Your Price:$329.00
In Stock.
Part Number:HMT-35-123

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H1-depleted mono/di-nucleosomes purified from HeLa cells. These are core nucleosomes comprising histone octamers (two copies each of histones H3, H4, H2A, H2B), each wrapped with ~146 bp of DNA with ~50 additional bp of internucleosomal DNA. Useful for the assay of various histone methyltransferases (e.g. MLL1 Complex, MLL2 Complex, MLL4 Complex and NSD2) by methods employing radiolabeling with [3H]-S-adenosylmethionine (e.g. gel electrophoresis/autoradiography or filterplate/scintillation counting).

NOTE: Each vial contains 50 µg nucleosomal DNA, determined by A260nm. At ~200 bp/nucleosome, the total weight, DNA + protein, is thus ~91 µg.

A 50ug vial of RBC nucleosomes contains over 80% more product than those from vendors that sell by total weight.

SOURCE: HeLa S3 cells                

SUPPLIED AS: Purified HeLa nucleosomes in 20 mM HEPES pH 7.5, 1 mM EDTA, 0.5 mM PMSF, 1 mM ß-mercaptoethanol, 20% glycerol (w/v).

STORAGE: -80°C, aliquot and snap-freeze after first use.

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Substantial Discounts Available on Bulk Quantities        

With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

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