Oligonucleosomes purified from chicken blood cells by a modification of the method of Schnitzler. These are H5 containing core nucleosomes comprising histone octamers, each wrapped with ~146 bp of DNA with ~50 additional bp of internucleosomal DNA.

NOTE:Each vial contains 50 µg nucleosomal DNA, determined by A260nm. At ~200 bp/nucleosome, the total weight, DNA + protein, is thus ~91 µg.

A 50ug vial of RBC nucleosomes contains over 80% more product than those from vendors that sell by total weight.

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted portion should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted solutions is not recommended.