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Nucleosomes (Chicken Mono/Di)

Your Price:$308.00
In Stock.
Part Number:HMT-35-179

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**Please note that shipping and dry ice charges will be included in your final invoice.**


 Mono/di-nucleosomes purified from chicken blood cells. These are core nucleosomes comprising histone octamers (two copies each of histones H3, H4, H2A, H2B), each wrapped with ~146 bp of DNA with ~50 additional bp of internucleosomal DNA. Useful for the assay of various histone methyltransferases (e.g. MLL1 Complex, MLL2 Complex, MLL4 Complex, Dot1L and NSD2) by methods employing radiolabeling with [3H]-S-adenosylmethionine (e.g. gel electrophoresis/autoradiography or filterplate/scintillation counting). Most histone methyltransferases that require nucleosomes for assay have roughly equal or somewhat greater activity with chicken, relative to HeLa, nucleosomes.

NOTE: Each vial contains 50 µg nucleosomal DNA, determined by A260nm. At ~200 bp/nucleosome, the total weight, DNA + protein, is thus ~91 µg.

A 50ug vial of RBC nucleosomes contains over 80% more product than those from vendors that sell by total weight.

SOURCE: Chicken blood cells


STORAGE: -80°C, aliquot and snap-freeze after first use.

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Substantial Discounts Available on Bulk Quantities                

With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

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