Nuclear Extract (HeLa) is a high salt extract of nuclei from HeLa-S3 cells (human cervical cancer line). Such extracts contain various enzymes and multi-enzyme complexes with epigenetic modification activities, such as histone deacetylases (HDACs) and histone methyltransferases (HMTs). RBC’s HeLa Nuclear Extract displays histone methyltransferase (HMT) activity in radiolabeled assays with [3H]-S-adenosylmethionine and core histones or nucleosomes as substrates and deacetylase (HDAC) activity with a fluorogenic acetylated lysine substrate.
SUPPLIED AS: Dialyzed and clarified protein extract at 7-10 mg/mL in 20 mM HEPES/NaOH pH 7.9, 100 mM KCl, 0.2 mM EDTA, 0.5 mM PMSF, 0.5 mM DTT, 20% glycerol (v/v).
STORAGE: -80°C, aliquot and snap-freeze after first use.
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