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Nuclear Extract (HeLa)

Your Price:$83.00
In Stock.
Part Number:HMT-30-136

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DESCRIPTION:
Nuclear Extract (HeLa) is a high salt extract of nuclei from HeLa-S3 cells (human cervical cancer line).   Such extracts contain various enzymes and multi-enzyme complexes with epigenetic modification activities, such as histone deacetylases (HDACs) and histone methyltransferases (HMTs).   RBC’s HeLa Nuclear Extract displays histone methyltransferase (HMT) activity in radiolabeled assays with [3H]-S-adenosylmethionine and core histones or nucleosomes as substrates and deacetylase (HDAC) activity with a fluorogenic acetylated lysine substrate. 

SOURCE: HeLa S3 cells

SUPPLIED AS: Dialyzed and clarified protein extract at 7-10 mg/mL in 20 mM HEPES/NaOH pH 7.9, 100 mM KCl, 0.2 mM EDTA, 0.5 mM PMSF, 0.5 mM DTT, 20% glycerol (v/v).

STORAGE: -80°C, aliquot and snap-freeze after first use.

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With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

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