NSD2-T1150A is a mutant human recombinant NSD2 with alanine (A) substituted for threonine-1150 (T1150) and expressed in Sf9 insect cells. NSD2 is a SET-domain lysine methyltransferase that exerts regulatory effects primarily through methylation of H3K36, a mark associated with gene activation. NSD2 may also act as an H4K20 methyltransferase as part of a DNA damage response. Notable for its role in the genesis of t(4; 14) translocation-positive multiple myelomas, NSD2 is also overexpressed in a wide range of human cancers, including breast, lung and colon cancers. The T1150A mutation, along with E1099K, a prevalent mutation in pediatric acute lymphoblastic leukemia (ALL), are found recurrently in mantle cell lymphomas (MCL). A truncated E1099K construct (residues 955-1365) has been reported to exhibit elevated methylation activity toward nucleosomes in vitro and the same appears to be the case with full-length NSD2-T1150A (see data sheet).
ACCESSION #: NM_001042424
INCLUDES AMINO ACIDS: 2-1365
TAG(S): N-terminal His tag
MW: 155.5 kDa
EXPRESSION SYSTEM: Insect cell/Baculovirus
SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris/HCl pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v).
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted enzyme should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted enzyme is not recommended.
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