customer service

NSD2-T1150A Methyltransferase

Your Price:$495.00
In Stock.
Part Number:HMT-21-181

Choose Options


**Please note that shipping and dry ice charges will be included in your final invoice.**

NSD2-T1150A is a mutant human recombinant NSD2 with alanine (A) substituted for threonine-1150 (T1150) and expressed in Sf9 insect cells. NSD2 is a SET-domain lysine methyltransferase that exerts regulatory effects primarily through methylation of H3K36, a mark associated with gene activation. NSD2 may also act as an H4K20 methyltransferase as part of a DNA damage response.  Notable for its role in the genesis of t(4; 14) translocation-positive multiple myelomas, NSD2 is also overexpressed in a wide range of human cancers, including breast, lung and colon cancers. The T1150A mutation, along with E1099K, a prevalent mutation in pediatric acute lymphoblastic leukemia (ALL), are found recurrently in mantle cell lymphomas (MCL). A truncated E1099K construct (residues 955-1365) has been reported to exhibit elevated methylation activity toward nucleosomes in vitro and the same appears to be the case with full-length NSD2-T1150A (see data sheet).

ACCESSION #: NM_001042424

Uniprot Link


TAG(S): N-terminal His tag

MW: 155.5 kDa

EXPRESSION SYSTEM: Insect cell/Baculovirus

SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris/HCl pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v).

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted enzyme should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted enzyme is not recommended.

View Product Data Sheet

Substantial Discounts Available on Bulk Quantities

We also offer assay services for NSD2-T1150A.

With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

Reaction Biology Corp. 1 Great Valley Parkway, Suite 2 Malvern, PA 19355 1-877-347-2368 or 610-722-0247
[email protected]
Reaction Biology Corp. © 2013 all rights reserved.