Human recombinant NSD2. Catalyzes the transfer of methyl groups from S-adenosyl-L-methionine (SAM) to the e-amino function of protein L-lysine residues, specifically lysine-36 of histone H3 (H3K36)1 with, depending on substrate, activities also reported at H3K42, H4K202 and H4K441 (see also review3). NSD2’s H4K20 methylation may be linked to double strand breaks and the DNA damage response4, whereas its principal regulatory functions appear to occur via methylation of H3K365,6, a mark associated with active transcription. NSD2 is overexpressed in multiple myelomas with the t(4;14) translocation7. NSD2 knockdown in such cells induces apoptosis5, while overexpression of catalytically active NSD2 promotes oncogenic transformation and tumor formation even in the absence of the translocation6. In addition to t(4;14)+ multiple myelomas, NSD2 expression is elevated in a variety of cancers (bladder8,9, breast8, prostate8, kidney8, lung8,9, pancreas8, colon9, stomach9, anal canal9, female genitals9, skin9, neuroblastoma10) and its carcinogenic effects may be mediated by interaction with ß-catenin and effects on the WNT pathway8. Consequently, NSD2 is eliciting strong interest as a possible target for anti-cancer therapeutics3,8.
ACCESSION #: NM_001042424
INCLUDES AMINO ACIDS: 934-1241
TAG(S): N-terminal His-tag
MW: 37.4 kDa
EXPRESSION SYSTEM: E. coli
SUPPLIED AS: µM NSD2 ( µg/µl total protein) in 50 mM Tris/HCl pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v).
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted enzyme should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted enzyme is not recommended.View Product Data Sheet
Available on Bulk Quantities