MLL1 Complex is a complex catalyzing the transcription-activating methylation of histone H3 lysine-4 and comprising a catalytic, SET domain-containing subunit, MLL1, plus the subunits of an activity-enhancing sub-complex—WDR5, RbBP5, Ash2L and DPY-30 (2 copies). MLL1 gene rearrangements that include the SET domain (partial tandem duplications) occur in 5-10% of acute myeloid leukemias (AMLs) and wild-type MLL1 can also be required for leukemic transformation by MLL1 fusion proteins, suggesting inhibition of MLL1 methyltransferase activity as a strategy for anti-leukemia therapy.
ACCESSION #: NM_005933, NM_017588, NM_005057, NM_001105214, NM_0325742
INCLUDES AMINO ACIDS: MLL1 3745-3969 (C-term.), WDR5 22-334 (C-term.), RbBP5 1-538 (C-term.), Ash2L 2-534 (C-term.), DPY-30 1-99 (C-term.)
TAG(S): N-terminal His-tag on all subunits
MW: 212.0 kDa
EXPRESSION SYSTEM: E. coli
SUPPLIED AS: Solution of purified recombinant proteins in 20 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM TCEP (tris(2-carboxyethyl)phosphine HCl), 10% (w/v) glycerol, 1 µM ZnCl2.
STORAGE: -80°C, aliquot and snap-freeze after first use.
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