Full-length, untagged human recombinant histone H3.3. Histone H3.3 is incorporated into chromatin independently of replication and is enriched in areas of active gene transcription. RBC’s Histone H3.3 is useful for the assay of various histone methyltransferases (e.g. MLL2 Complex) either by radiolabeling with [3H]-S-adenosylmethionine (e.g. gel electrophoresis/autoradiography or filterplate/scintillation counting) or by methods employing site/modification-specific antibodies (e.g. anti-H3K4me2).
ACCESSION #: NM_002107
INCLUDES AMINO ACIDS: 1-136
MW: 15.3 kDa
EXPRESSION SYSTEM: E. coli
SUPPLIED AS: Solution of purified recombinant protein in 50 mM HEPES/NaOH pH 7.1, 10 mM KCl, 1 mM DTT, 1 mM EDTA, 10% glycerol (v/v).
STORAGE: -80°C, aliquot and snap-freeze after first use.
View Product Data Sheet
Substantial Discounts Available on Bulk Quantities