Full-length, untagged human recombinant histone H2B expressed in E. coli. One of the four, core nucleosomal histones, H2B forms dimers with histone H2A, two of which associate with the H3-H4 tetramer (H3-H4)2, forming the core octamer of the nucleosome. Histone H2B is subject to several regulatory post-translational modifications including monoubiquitination, phosphorylation, acetylation, lysine methylation and crotonylation. Monoubiquitination of H2B lysine-120 (H2BK120ub) stimulates H3K4 methylation in chromatin, an effect which recent evidence suggests is mediated in trans via ubiquitin binding by Ash2L, a component of the human MLL/SET1 family methyltransferase complexes. Monoubiquitination at both H2BK34 and H2BK120 has been shown to enhance H3K79 methylation by Dot1L. RBC’s Histone useful for the assay of histone acetyltransferases (HATs; e.g. p300, CBP) either by radiolabeling with [3H]-S-adenosylmethionine (e.g. gel electrophoresis/autoradiography or filterplate/scintillation counting) or by non-radiolabeled methods (e.g. detection with site-specific anti-acetyllysine antibodies).
ACCESSION #: NM_0080593
INCLUDES AMINO ACIDS: 1-126
MW: 13.9 kDa
EXPRESSION SYSTEM: E. coli
SUPPLIED AS: Solution of purified recombinant protein in 50 mM HEPES/NaOH pH 7.1, 10 mM KCl, 1 mM DTT, 1 mM EDTA, 10% glycerol (v/v).
STORAGE: -80°C, aliquot and snap-freeze after first use.
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