Full-length, untagged human recombinant histone H1.0 expressed in E. coli. H1 histones function as ‘linkers’ that bind to internucleosomal DNA (~1:1 with the histone octamer of the nucleosome core), converting nucleosomes to “chromatosomes” and promoting the formation of more compact, higher-order chromatin structure. Histone H1.0, the H1 variant most homologous to the H5 histone of avian erythrocytes, is, along with histones H1.1-H1.5, one of six H1 variants found in terminally differentiated cells. Recent work indicates that aside from playing a role in chromatin structure and function, histone H1.0 interacts with a wide array of nucleolar proteins, with specific interactions implying a role in rRNA splicing and ribosome biogenesis. In general, phosphorylation is the predominant H1 post-translational modification, notably associated with cell cycle progression, but other modifications, including lysine acetylation/methylation1 and ADP-ribosylation10, are also reported. Crosstalk occurs between H1 modifications and those on other histones and H1 variants can affect particular histone modifications, e.g. H1.3 (mouse H1d) inhibition of SET7/9 H3K4 methylation (see data sheet), consistent with a role for H1 in transcriptional regulation. Indeed, gene-specific transcriptional regulatory effects of H1 variants have been reported, but these remain less well studied than H1’s role in chromatin structure.
ACCESSION #: NM_005318
INCLUDES AMINO ACIDS: 1-194
MW: 20.9 kDa
EXPRESSION SYSTEM: E. coli
SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris/HCl pH 7.5, 750 mM NaCl, 1 mM TCEP, 10% glycerol (v/v).
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.
STORAGE: -80°C, aliquot and snap-freeze after first use.
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