Mutant human recombinant HRAS (G12V) with valine (V) substituted for glycine-12 (G12)
ACCESSION #: NP_005334.1
INCLUDES AMINO ACIDS: residues 2-169
TAG(S): N-terminal His-fusion protein
MW: 21.4 kDa
EXPRESSION SYSTEM: E. coli
PURITY: >90% by SDS-PAGE
SUPPLIED AS: Solution of purified recombinant protein in 25 mM Tris, pH 8.0, 500 mM NaCl, 1 mM TCEP, 5 mM MgCl2, .
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry ice/ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted enzyme is not recommended.
