HDAC6, catalyzes the deacetylation of protein acetyllysine residues and, along with HDAC10, is classified as a class IIb HDAC. Class II HDACs, like class I (HDACs 1-3, 8) and class IV (HDAC11) enzymes are Zn(II)-dependent amidohydrolases, mechanistically and phylogenetically distinct from the NAD+-dependent sirtuin deacetylases (class III HDACs). HDAC6 is unique in that it comprises two full HDAC catalytic domains. Primarily a cytoplasmic enzyme, HDAC6 can form a complex with SIRT2 and act as a tubulin deacetylase. Aside from its HDAC domains, HDAC6 contains a dynein interaction domain and a zinc-finger domain that binds the unconjugated C-termini of ubiquitin found in protein aggregates. HDAC6 functions in multiple stress-response pathways for alleviating the effects of misfolded and aggregated proteins, including aggresome formation, autophagy, the positive regulation of Hsp90 chaperone activity and the activation of HSF1 and consequent increased expression of additional chaperones. HDAC6 activity can promote oncogenesis and selective HDAC6 inhibition, perhaps especially in combination with DNA damaging agents, is considered a promising approach for anti-cancer therapy. Evidence for cigarette smoke-induced and HDAC6-dependent autophagic shortening of cilia in airway epithelia has led to the suggestion of HDAC6 inhibition as a possible therapeutic approach for COPD. There is evidence that HDAC6 inhibition might be beneficial for some neurodegenerative disorders including Alzheimer’s disease. However, in other neurodegenerative disease model systems, HDAC6 overexpression confers benefits, as might be expected from its role in promoting the elimination of protein aggregates.
ACCESSION #: NM_006044
INCLUDES AMINO ACIDS: 1-1215
TAG(S): N-terminal GST-tag
MW: 158.3 kDa
EXPRESSION SYSTEM: Insect cell/Baculovirus
SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris/HCl pH 7.5, 500 mM NaCl, 10% glycerol (v/v)
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted enzyme should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted enzyme is not recommended.
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