customer service




EZH2-Y641F Complex

Your Price:$879.00
In Stock.
Part Number:HMT-25-173

Choose Options

Amount

**Please note that shipping and dry ice charges will be included in your final invoice.**

DESCRIPTION:
A five-member complex (EZH2-Y641F, AEBP2, EED, RbAp48, SUZ12) in which the catalytic subunit (EZH2-Y641F) is mutant human recombinant EZH2 with phenylalanine (F) substituted for tyrosine-641 (Y641). Acts as a histone H3 lysine-27 methyltransferase, generating trimethylated histone H3 lysine-27 (H3K27me3), an epigenetic mark essential for programmed repression of gene expression. EZH2 mutations at Y641, including Y641F, are associated with certain types of lymphoma in which they occur as heterozygotes with the wild-type allele.. While wild-type EZH2 is most efficient at monomethylation of unmethylated H3K27, the Y641F mutant has poor efficiency for this first methylation step but increased efficiency in transferring the second and third methyl groups. Thus the combined action of the mutant and wild-type enzyme produces the elevated levels of H3K27me3 observed in these lymphomas.

ACCESSION #: NM_001203247, NM_001114176, NM_003797, NM_005610, NM_015355

Uniprot Link

INCLUDES AMINO ACIDS: EZH2-Y641F 2-746, AEBP2 2-517, EED 2-441, RbAp48 2-425, SUZ12 2-739

TAG(S): N-terminal Flag-tag on EED, all others N-terminal His-tag

MW: 333.8 kDa

EXPRESSION SYSTEM: Insect cell/Baculovirus

SUPPLIED AS: Solution of purified recombinant proteins in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol (w/v), 3 mM DTT.

STORAGE: -80°C, aliquot and snap-freeze after first use.

View Product Data Sheet

Substantial Discounts Available on Bulk Quantities

We also offer assay services for EZH2-Y641F.











With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

Reaction Biology Corp. 1 Great Valley Parkway, Suite 2 Malvern, PA 19355 1-877-347-2368 or 610-722-0247
[email protected]
Reaction Biology Corp. © 2013 all rights reserved.