A five-member complex (EZH2-Y641F, AEBP2, EED, RbAp48, SUZ12) in which the catalytic subunit (EZH2-Y641F) is mutant human recombinant EZH2 with phenylalanine (F) substituted for tyrosine-641 (Y641). Acts as a histone H3 lysine-27 methyltransferase, generating trimethylated histone H3 lysine-27 (H3K27me3), an epigenetic mark essential for programmed repression of gene expression. EZH2 mutations at Y641, including Y641F, are associated with certain types of lymphoma in which they occur as heterozygotes with the wild-type allele.. While wild-type EZH2 is most efficient at monomethylation of unmethylated H3K27, the Y641F mutant has poor efficiency for this first methylation step but increased efficiency in transferring the second and third methyl groups. Thus the combined action of the mutant and wild-type enzyme produces the elevated levels of H3K27me3 observed in these lymphomas.
ACCESSION #: NM_001203247, NM_001114176, NM_003797, NM_005610, NM_015355
INCLUDES AMINO ACIDS: EZH2-Y641F 2-746, AEBP2 2-517, EED 2-441, RbAp48 2-425, SUZ12 2-739
TAG(S): N-terminal Flag-tag on EED, all others N-terminal His-tag
MW: 333.8 kDa
EXPRESSION SYSTEM: Insect cell/Baculovirus
SUPPLIED AS: Solution of purified recombinant proteins in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol (w/v), 3 mM DTT.
STORAGE: -80°C, aliquot and snap-freeze after first use.
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