Human recombinant EP300 bromodomain. EP300 and the closely related CREBBP (CBP) are major regulators of gene transcription (co-activators) and function as lysine acetyltransferases for histones (i.e. HATs) and for numerous other proteins (e.g. p53). Aside from the acetyllysine-binding bromodomain and the catalytic HAT domain, native EP300 comprises multiple protein-protein interaction domains (3 Cys-His-rich (CH) domains, KIX domain, steroid receptor co-activator interaction domain (SID)) and also has E3 and E4 ubiquitin ligase activities.
ACCESSION #: NM_001429
INCLUDES AMINO ACIDS: 1040-1161
TAG(S): N-terminal His-tag
MW: 17.2 kDa
EXPRESSION SYSTEM: E. coli
SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v)
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.
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Substantial Discounts Available on Bulk Quantities
We also offer assay services for EP300 (His).