DNA (cytosine-5)-methyltransferase 3a (DNMT3a) is required for de novo DNA methylation and methylates at both CpG and non-CpG sites.
ACCESSION #: NM_175629
Uniprot Link
INCLUDES AMINO ACIDS: 214-912 (C-term.)
TAG(S): N-terminal GST-tag and C-terminal StrepII tag
MW: 107 kDa
EXPRESSION SYSTEM: Sf9 insect cells
SUPPLIED AS: 1.62 µg/µl total protein in 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 3 mM DTT, 0.05% Tween 20 as determined by OD280.
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. Freezing and storage of diluted enzyme is not recommended.
