Human recombinant BRDT, tandem construct comprising bromodomains 1 and 2, expressed with an N-terminal His tag. BRDT, like other human members of the BET family of chromatin-binding proteins (BRD2, BRD3, BRD4), comprises two bromodomains, protein modules that bind epsilon-N-acetyllysine residues. Mouse BRDT-1 (BRDT bromodomain 1) can bind simultaneously to two acetyllysine residues and, among the multiply acetylated histone tails tested, had the highest affinity for a histone H4 peptide acetylated at lysines 5 and 8 (H4K5AcK8Ac). Expression of BRDT is testis-specific and deletion of the mouse BRDT-1 causes abnormal spermatid development and sterility. Human recombinant BRDT, tandem construct comprising bromodomains 1 and 2, expressed with an N-terminal His-tag. BRDT’s functions in spermiogenesis include roles in broad, programmatic regulation of gene expression, mRNA splicing, chromatin remodeling, meiosis, formation of the chromocenter and post-meiotic genome repackaging. A three-month treatment of male mice with the BET family bromodomain inhibitor, JQ1, reversibly eliminated fertility, highlighting the potential of BRDT-specific inhibition as an approach for pharmacologic male contraception.
ACCESSION #: NM_001726
INCLUDES AMINO ACIDS: 21-383
TAG(S): N-terminal His tag
MW: 44.0 kDa
EXPRESSION SYSTEM: E. coli
SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris/HCl, pH 7.5, 150 mM NaCl, 1.0 mM TCEP, 10% glycerol (v/v)
STORAGE: -80°C, aliquot and snap-freeze after first use.
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