Human recombinant BRDT, bromodomain-2. BRDT, like other human members of the BET family of chromatin-binding proteins (BRD2, BRD3, BRD4), comprises two bromodomains, protein modules that bind e-N-acetyllysine residues. Expression of BRDT is testis-specific and deletion of the mouse BRDT-1 (bromodomain 1) causes abnormal spermatid development and sterility. BRDT’s functions in spermiogenesis include roles in broad, programmatic regulation of gene expression, mRNA splicing, chromatin remodeling, meiosis, formation of the chromocenter and post-meiotic genome repackaging. A three-month treatment of male mice with the BET family bromodomain inhibitor, JQ1, reversibly eliminated fertility, highlighting the potential of BRDT-specific inhibition as an approach for pharmacologic male contraception. The structure of the mouse BRDT-2 in complex with a histone H3 K18Ac peptide has been determined. The experimental atherosclerosis drug and BET family bromodomain inhibitor RVX-208 is selective for the second bromodomains of this group, including BRDT-2.
ACCESSION #: NM_001242806
INCLUDES AMINO ACIDS: 272-383
TAG(S): N-terminal His-tag
MW: 18.6 kDa
EXPRESSION SYSTEM: E.coli
SUPPLIED AS: Solution of purified recombinant protein in 50 mM HEPES/NaOH, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.
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Substantial Discounts Available on Bulk Quantities
We also offer assay services for BRDT-2 (His).