Human recombinant BRD9 bromodomain. BRD9 has been identified as a subunit of the BAF-subtype of SWI/SNF nucleosome remodeling complexes. The SWI/SNF complexes are the most highly mutated chromatin regulator in human cancers (19.6% in 44 published sequencing studies), although BRD9 subunit is among the least mutated subunits. However, increased copy numbers of the BRD9 gene occur with high frequency in early stage non-small cell lung cancers and in invasive cervical cancers. The BRD9 bromodomain was found to bind, in vitro, to various microarrayed histone H3, singly lysine-acetylated peptides.
ACCESSION #: NM_023924
INCLUDES AMINO ACIDS: 135-248
TAG(S): N-terminal His-tag
MW: 15.9 kDa
EXPRESSION SYSTEM: E. coli
SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v)
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.
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Substantial Discounts Available on Bulk Quantities
We also offer assay services for BRD9 (His).