Human recombinant BRD4, (residues 1-744), like other human members of the BET family of chromatin-binding proteins (BRD2, BRD3, BRDT), comprises two bromodomains, protein modules that bind e-N-acetyllysine residues. The ubiquitously expressed BRD4 functions as a transcriptional regulator with roles in cell cycle progression and has recently been shown to be an atypical kinase that can phosphorylate RNA Pol II. Recent structural studies have shown that BRD4-1, like the bromodomain-1 of fellow BET family protein BRDT, can bind simultaneously to two acetyllysine residues with appropriate spacing and sequence context, for example a histone H4 peptide acetylated at lysines 5 and 8 (H4K5AcK8Ac). Chromosomal translocations that produce BRD4-NUT fusion proteins are implicated in causation of a rare and aggressive cancer, NUT midline carcinoma. Selective inhibitors of BRD4/BET family bromodomains are showing promise as possible therapeutic agents for cancer and inflammation.

ACCESSION #: NM_058243

Uniprot Link

INCLUDES AMINO ACIDS: 1-744

TAG(S): N-terminal StrepII-tag and C-terminal His-tag

MW: 85.7 kDa

EXPRESSION SYSTEM:Sf9 insect cells

SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris/HCl, pH 8, 500 mM NaCl, 10% glycerol (v/v)

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted enzyme is not recommended.