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BRD3-Tndm (GST)

Your Price:$303.00
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Part Number:RD-11-166

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DESCRIPTION:
Human recombinant BRD3, tandem construct comprising bromodomains 1 and 2, expressed as an N-terminal GST fusion protein. BRD3, like other human members of the BET family of chromatin-binding proteins (BRD2, BRD4, BRDT), comprises two bromodomains, protein modules that bind epsilon-N-acetyllysine residues.  Recent results suggest an important role for BRD3 in linking acetylation of both histones and non-histone proteins to gene transcription.  When overexpressed in 293 cells, BRD3, along with BRD2, binds the hyperacetylated chromatin of transcribed genes, regions enriched in acetylated histone H4 lysine-5 (H4K5Ac), H4K12Ac, H3K14Ac, but deficient in H4K16Ac and H3K9me.  In an in vitro RNA polymerase II transcription system, binding of either BRD3 or BRD2 to a chromatin template assembled with hyperacetylated histones enabled transcription through the nucleosomes.  Although BRD3-2 (BRD3 bromodomain 2) has been shown to bind tetracetylated histone H4 tail sequence (H4K5AcK8AcK12AcK16Ac), the interaction is weaker than that of BRD3-1.  The BET family inhibitor, I-BET151, has shown efficacy in mouse models of MLL-fusion leukemias, displacing BRD3 and BRD4 from chromatin and inhibiting transcription of genes, e.g. BCL2, upregulated by the MLL-fusions. 

ACCESSION #: NM_007371

Uniprot Link

INCLUDES AMINO ACIDS: 1-434

TAG(S): GST

MW: 74.4 kDa

EXPRESSION SYSTEM: E. coli

SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris/HCl, pH 7.5, 500 mM NaCl, 1.0 mM TCEP, 10% glycerol (v/v).

STORAGE: -80°C, aliquot and snap-freeze after first use.

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