customer service

BRD3-1 (His)

Your Price:$303.00
In Stock.
Part Number:RD-11-143

Choose Options


**Please note that shipping and dry ice charges will be included in your final invoice.**

BRD3, like other human members of the BET family of chromatin-binding proteins (BRD2, BRD4, BRDT), comprises two bromodomains, protein modules that bind epsilon-N-acetyllysine residues.  Recent results suggest an important role for BRD3 in linking acetylation of both histones and non-histone proteins to gene transcription.  When overexpressed in 293 cells, BRD3, along with BRD2, binds the hyperacetylated chromatin of transcribed genes, regions enriched in acetylated histone H4 lysine-5 (H4K5Ac), H4K12Ac, H3K14Ac, but deficient in H4K16Ac and H3K9me.  In an in vitro RNA polymerase II transcription system, binding of either BRD3 or BRD2 to a chromatin template assembled with hyperacetylated histones enabled transcription through the nucleosomes.  In addition to acetylated histones, BRD3-1 (BRD3 bromodomain 1) has been found to bind the hematopoietic transcription factor GATA1 and to enhance its chromatin binding and activation of target genes.  The BET family inhibitor, I-BET151, has shown efficacy in mouse models of MLL-fusion leukemias, displacing BRD3 and BRD4 from chromatin and inhibiting transcription of genes, e.g. BCL2, upregulated by the MLL-fusions. 

ACCESSION #: NM_007371

Uniprot Link


TAG(S): N-terminal His-tag

MW: 17.0 kDa


SUPPLIED AS: Solution of purified recombinant protein in 10 mM HEPES/NaOH, pH 7.5, 150 mM NaCl, 0.5 mM TCEP

STORAGE: -80°C, aliquot and snap-freeze after first use.

View Product Data Sheet

Substantial Discounts Available on Bulk Quantities

We also offer assay services for BRD3-1 (His).

With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

Reaction Biology Corp. 1 Great Valley Parkway, Suite 2 Malvern, PA 19355 1-877-347-2368 or 610-722-0247
[email protected]
Reaction Biology Corp. © 2013 all rights reserved.