customer service




BRD2-2 (His)

Your Price:$303.00
In Stock.
Part Number:RD-11-145

Choose Options

Amount

**Please note that shipping and dry ice charges will be included in your final invoice.**

DESCRIPTION:
BRD2, like other human members of the BET family of chromatin-binding proteins (BRD3, BRD4, BRDT), comprises two bromodomains, protein modules that bind epsilon-N-acetyllysine residues. When overexpressed in 293 cells, BRD2, along with BRD3, binds the hyperacetylated chromatin of transcribed genes, regions enriched in acetylated histone H4 lysine-5 (H4K5Ac), H4K12Ac, H3K14Ac, but deficient in H4K16Ac and H3K9me. A single H4K5AcK12Ac peptide can bind two copies of BRD2-2 (BRD2, bromodomain 2), each interacting with one of the two acetylated lysines. In an in vitro RNA polymerase II transcription system, binding of either BRD2 or BRD3 to a chromatin template assembled with hyperacetylated histones enabled transcription through the nucleosomes. Further, BRD2 displayed histone chaperone activity, catalyzing the transfer of histone octamers from hyperacetylated oligonucleosomes to a labeled 190 bp 5s rDNA fragment. Like BRD4, BRD2 is a ubiquitously expressed transcriptional regulator and atypical protein kinase, with functions in cell cycle progression and embryogenesis. BRD2 binds preferentially to hyperacetylated histone H4 in H2A.Z-containing nucleosomes and this interaction is required for activation of androgen receptor (AR)-regulated genes in prostate cancer cells. In addition to prostate cancer, leukemia is a potential indication for specific BRD2 inhibition. BRD2 suppresses HIV transcription in latently infected cells and may therefore represent a target in therapeutic strategies involving viral reactivation. 

ACCESSION #: NM_005104

Uniprot Link

INCLUDES AMINO ACIDS: 344-454

TAG(S): N-terminal His-tag

MW: 15.7 kDa

EXPRESSION SYSTEM: E. coli

SUPPLIED AS: Solution of purified recombinant protein in 20 mM Tris pH 7.5, 150 mM NaCl, 1.0 mM TCEP, 10% glycerol (v/v) as determined by OD280

STORAGE: -80°C, aliquot and snap-freeze after first use.

View Product Data Sheet

 

Substantial Discounts Available on Bulk Quantities

We also offer assay services for BRD2-2 (His).











With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

Reaction Biology Corp. 1 Great Valley Parkway, Suite 2 Malvern, PA 19355 1-877-347-2368 or 610-722-0247
Sales@reactionbiology.com
Reaction Biology Corp. © 2013 all rights reserved.