Human recombinant BRD1 bromodomain. Native BRD1 (BRPF-2) contains, in addition to its acetyllysine-binding bromodomain, two other histone binding domains—a PWWP domain (histone H3 K79me2/3 & K36me3 binding) and a PHD zinc-finger (PHD1, N-terminal tail, unmodified histone H3 binding)—as well as a DNA binding domain (PHD2). Although assumed, like fellow family members BRPF-1 and BRPF-3 to perform a scaffolding function as part of MOZ/MORF histone acetyltransferase (HAT) complexes, BRD1 has been shown to form a complex with another MYST-family HAT, HBO1, plus ING4, and to be required for H3K14 acetylation and erythropoiesis.
ACCESSION #: NM_014577
INCLUDES AMINO ACIDS: 556-688
TAG(S): N-terminal His-tag
MW: 18.2 kDa
EXPRESSION SYSTEM: E. coli
SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris-HCl pH 7.5, pH 7.0, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v)
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.
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Substantial Discounts Available on Bulk Quantities
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