Human recombinant BPTF bromodomain construct. Full-length BPTF1 is a DNA and histone-binding component of the NURF nucleosome remodeling complex. This construct comprises C-terminal bromodomain, which has binding affinity for various histone tail acetyllysines, including H4 K5Ac, K12Ac and K16Ac. BPTF expression is elevated in developing neurons, but also in neuronal tissue under various neurodegenerative conditions. Amplification of the BPTF-coding chromosomal locus is prevalent in various cancers and knockdown of BPTF can restrict proliferation in cultured cells with an engineered pre-malignant phenotype.
INCLUDES AMINO ACIDS: 2791-2920 (C-term.)
TAG(S): N-terminal His tag
MW: 17.9 kDa
EXPRESSION SYSTEM: E.coli
SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v)
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.
View Product Data Sheet
Substantial Discounts Available on Bulk Quantities
We also offer assay services for BPTF-[BRD](His).