Human recombinant BAZ1B bromodomain. BAZ1B (WSTF) is a key subunit of several ATP-dependent chromatin remodeling complexes with activities in nucleosome and chromatin assembly, in transcriptional regulation (at vitamin D-receptor (VDR) target genes) and in DNA replication and repair. The full-length protein comprises multiple domains, including the N-terminal domain which confers the atypical tyrosine kinase activity responsible for H2A.X Tyr142 phosphorylation, a key regulatory mark in the DNA damage response. The BAZ1B bromodomain displays affinity for several acetylated histone tail peptides, particularly ones incorporating H3K14(Ac). Interaction between BAZ1B and the gene promoter for the vitamin D synthetic enzyme 25(OH)D3 1a-hydroxylase, most likely mediated by bromodomain binding to acetylated histones, is necessary for ligand-dependent transcriptional repression by VDR. By virtue of its regulatory role in aromatase gene expression, BAZ1B may hold potential as a target for therapy in estrogen-dependent breast cancer.
ACCESSION #: NM_032408
INCLUDES AMINO ACIDS: 1340-1457
TAG(S): C-terminal His- and Strep-tags
MW: 17.1 kDa
EXPRESSION SYSTEM: E. coli
SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v)
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.
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Substantial Discounts Available on Bulk Quantities
We also offer assay services for BAZ1B (His).