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ATAD2B (GST)

Your Price:$303.00
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Part Number:RD-11-185

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DESCRIPTION:
Human recombinant ATAD2B bromodomain. In addition to a bromodomain, native ATAD2B contains an AAA ATPase domain. Expressed and located to the nucleus during neuronal development (chick embryos), ATAD2B is expressed at elevated levels in some human tumors (glioblastoma, oligodendroglioma, breast cancer), but is present in the cytoplasm or membrane-bound fractions. The ATAD2B bromodomain binds various Lys(Ac) residues in singly acetylated histone peptide microarrays (histones H1.4, H2A, H2B, H3, H4) with binding to histone H4 K5(Ac) confirmed in solution by isothermal titration calorimetry (ITC).

ACCESSION #: NM_017552

Uniprot Link

INCLUDES AMINO ACIDS: 952-1086

TAG(S): N-terminal GST-tag

MW: 42.6 kDa 

EXPRESSION SYSTEM: E. coli

SUPPLIED AS: Solution of purified recombinant protein in 50 mM HEPES/NaOH, pH 7.0, 500 mM NaCl, 1 mM TCEP, 10 % glycerol

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted enzyme is not recommended.

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