customer service

ASH1L (His) Methyltransferase

Your Price:$358.00
In Stock.
Part Number:HMT-11-376

Choose Options


**Please note that shipping and dry ice charges will be included in your final invoice.**


ASH1L is a large , multi-domain protein associated with actively transcribed regions of chromatin, ASH1L is the human homolog of Drosophila Ash1, a Trithorax group protein. Like its counterpart in Drosophila, ASH1L contains a SET histone methyltransferase domain and has been found to play a role in the regulation of Hox gene expression. Although the ASH1L SET domain has been shown in vitro to methylate histone peptides on lysine-4 of histone H3 (H3K4), ASH1L is an H3K36 methyltransferase both in vivo and in vitro with nucleosomes as substrate. Recruitment of ASH1L by the ncRNA DBE-T to the chromosome 4q35 locus associated with FSHD (facioscapulohumeral muscular dystrophy) leads to increased H3K36me2 and inappropriate gene derepression at the FSHD locus. In a possible positive feedback loop, ASH1L increases expression of DBE-T itself, suggesting ASH1L’s methyltransferase activity or its interaction with DBE-T as potential therapeutic targets for FSHD. RBC’s ASH1L comprises the catalytic domain (AWS/SET/Post-SET) fused to GST.

ACCESSION #: NM_018489 

Uniprot Link


TAG(S): N-terminal His-tag

MW: 35.4 kDa


SUPPLIED AS: Solution of purified recombinant protein in 100mM HEPES, pH 7.1, 500mM NaCl, 0.5 mM TCEP, 5% (v/v) glycerol

STORAGE: -70°C, Thaw quickly and store on ice before use. The remaining, unused, undiluted enzyme should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 ul) or storage of diluted enzyme is not recommended. 

View Product Data Sheet


Substantial Discounts Available on Bulk Quantities     

With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 590 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

Reaction Biology Corp. 1 Great Valley Parkway, Suite 2 Malvern, PA 19355 1-877-347-2368 or 610-722-0247
[email protected]
Reaction Biology Corp. © 2013 all rights reserved.