Human recombinant ASH1L bromodomain. ASH1L is a large (333 kDa), multi-domain protein associated with actively transcribed regions of chromatin. Its bromodomain lies C-terminal to its SET domain, which confers histone H3K36 methyltransferase activity. ASH1L is the human homolog of Drosophila Ash1, a Trithorax group protein. Like its counterpart in Drosophila, ASH1L has been found to play a role in the regulation of Hox gene expression. The ASH1L bromodomain displays strong binding to various Lys(Ac) residues in singly acetylated histone peptide microarrays (histones H1.4K74Ac, H2AK36Ac, H2BK85Ac, H3K56Ac, H4K59Ac/K79Ac).
ACCESSION #: NM_018489
INCLUDES AMINO ACIDS: 2428-2559
TAG(S): N-terminal His-tag
MW: 17.7 kDa
EXPRESSION SYSTEM: E. coli
SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v)
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.
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Substantial Discounts Available on Bulk Quantities